Hemacytometer



May 24 1927.

S. TRENNER HEIACYTOMETER Filed Aug. 25, 1925 Patented Mey v24, 192?.

UNITED STATES PATENT oFFlcr..

f sIMEoN TRENNER, for" rHILAnELPHrA, PENNSYLVANIA, assmnon 'ro 'ma FIRM or c. A. nAUssER eson, or rrrnianntri-na, PENNSYLVANIA, consisrrne or CARL; ADOLPH Hanssen AND anunciar Anon-.ent Hanssen.

HEMACYTOMETER.

fvApplication filed August 25,' 1925. Serial No. 52,366.

l rIhis invention relates especially 'to hemacytometer counting` chambers and rulings, and the principal object of the invention is `toprovide novel orienting means facilitating finding and safelyfocusing the ruling ofthe counting chamber under the microscope. e

In the attached drawing: Figure l is a plan view of a h'aemacyto meter countingchamber made in accordance with my invention;

Fig. 2 is a section on -the line 2-2,' Fig. l, and

Fig. 3 is a plan view of another form of haemacytometer also embodying my inven tiOIl. 1

l/Vith reference to Figs. l and 2 ofV the drawing, the haemacytometer counting cham` ber comprises in the present instance two por` tions l and 2 Whose surfaces are polished and' on Which the rulings 3 of the counting chambers are situated. of these portions l and 2, `I provide an area 4L having a matte or unpolished finislnand from each of these unpolished areas I eX- tend a finding line 5 toward the respective rulings 3, 3.

I prefer to form the matte surfaces 4: in a plane slightly ybelen7 the plane of the rulings 3, and the finding line 5 which may be engraved is of sufficient length, width and depth to render it conspicuous and promptly locat-able through the microscope,

In practice,l the low power objective may first be focusedy on one of lthe matte surfaces 4. The finding line 5 Vis then located and followed in the direction of the ruling 3 by moving the slidein the same plane by means of the fingers or mechanical stage, With the result that the ruling is located With,\ promptness and certainty. By reason of, the approximate coincidence in the plane of the matte surface 4 and the rulings l 3 and of therefraction of the emergent light pencils due to the interposition ofdiluting fluid `in the countingchamber, it will then be necessary to-foous up, i. e., raise the objective instead of lowering it as is usual Where no such focusing surface is utilized, to criti` cally sharpen the approximately focused image vof, the cells and rulings simultaneously. By this construction and method of procedure, the danger of disturbing the dis-- At the outer ends of each tribut-ion of cells `and vitiating the count by touching the cover glass with themicroscope objective is obviated, as also thek danger v of injuring the objective, cover glass or" ruling.`

There may be considerable variation in the embodiments of the invention, and in Fig. 3 I have shown a counting chamber of different type from that shown in Figs. l

and 2, to which my invention has been i applied. In this case, the matte surfaces 4 uponwhich the initial focus is made are separate from the polished surfaces upon which the rulings 3 are situated. Each of the polished surfaces, however, is provided With a finding line 5 extendingtowards the rulings 3, and the vprocedure is in all essential respects the same as that described .in connectionWiththe modification shown in Figs. l and 2. After focusingion the outer surface 4L, the slide is moved until the line 5 comes Within the field of vision, after Which the rulings are'easily located by moving the slide inthe direction indicated by the iinder. 4Here also the matte surfaces 4 are in approximate coincidence With the plane of the rulings 3 and are preferably slightly lower. f

I claim:

l. A haemacytometer counting chamber including a ruled area, a'clearlyy defined and immediately distinguishable surface for focusing7 and distinctive guide means eX- tending from said surface in the direction of said ruling.

2. A haemacytom'eter counting chamber including a ruled area, Well defined and distinctive guidemeans extending from said ruling, and a clearlyv defined and immedi` ately distinguishable focusing surface.

8. Al haemacytometer counting chamber including a ruled area and guide means in the form of a marking extending inthe direction of said ruling, said marking being Well defined and differing fromy the other markings of the. device so as to be readily distinguishable.

#LA haemacytometer counting chamber including a. ruled area and a single line extending as a guidein the direction of said area, said line "being Well defined and distinctive in appearance so as to be readily distinguishable.

5. A lifemiioytmnel'er counting clnnniivr Qmnprining' u ruld :uvm n, (nearly defined forming Suriiuo. und disljncl'ir guide lueur@ nefrnnmliiii'n mid siren und Suid surfiifa.

(E. i inrlu? zA ifurusi man whose plane Substantially (f0- infidvf: will] (lue plan@ 0l Suid ruling-rz :uid iilfzincive guide means inl'crlnodieil'e, mid ,Quil'ncefn T. A linnicylomeier counting lnunbor inCludiug 1 i'ruuFJliceni; nwniler. :l ymlirrlni-d surface` rulings imirkwi un smid rurfiufiu :i matte sur'fxico in a plane subsi'nntirilly Coin Ciding with the plane of said rulings, und a 1 ruim armi, :L rioni-ly airline-Li diminutive lin@l intermediate Suid surfaces.

E). lnmimcyloluvtir counting rlnuulwr cmnprisingf :i rulud :1i-0:1. :iu uumlr-d :irezi mirroiu'ulinff Suid ruled :um :uid well delimd :uni disini'iivu guide nnzulrll amending: :irrms tlm said unrulwl :arr-:i in lluA dirci:- tiwn olf said ruling.

SMEON IRENE-YER. 

